Article
Universal quenching probe system: flexible, specific, and cost-effective real-time polymerase chain reaction method.
Analytical chemistry - 15 Jul 2009
Tani Hidenori, Miyata Ryo, Ichikawa Kouhei, Morishita Soji, Kurata Shinya, Nakamura Kazunori, Tsuneda Satoshi, Sekiguchi Yuji, Noda Naohiro
Abstract excerpt
We have developed a flexible, specific, and cost-effective real-time polymerase chain reaction (PCR) method. In this technique, a quenching probe (QProbe) and a nonfluorescent 3'-tailed probe are used. The QProbe is a singly labeled oligonucleotide bearing a fluorescent dye that is quenched via electron transfer between the dye and a guanine base at a particular position. The nonfluorescent 3'-tailed probe...
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