Article
Species-specific and mutant MWFE proteins. Their effect on the assembly of a functional mammalian mitochondrial complex I.
The Journal of biological chemistry - 14 Jun 2002
Yadava Nagendra, Potluri Prasanth, Smith Erin N, Bisevac Amina, Scheffler Immo E
Abstract excerpt
The MWFE protein (70 amino acids) is highly conserved in evolution, but the human protein (80% identical to hamster) does not complement a null mutation in Chinese hamster cells. We have identified a small protein segment where significant differences exist between rodents and primates, illustrating very specifically the need for compatibility of the nuclear and mitochondrial genomes in the assembly of complex I....
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Blotting, Western
- CHO Cells
- Cricetinae
- DNA, Complementary
- Electron Transport Complex I
- Electrophoresis, Polyacrylamide Gel