Secretory production and purification of functional full-length streptavidin from Bacillus subtilis.

doi:10.1006/prep.2001.1582

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Article

Secretory production and purification of functional full-length streptavidin from Bacillus subtilis.

Protein expression and purification - 1 Apr 2002

Wu Sau-Ching, Hassan Qureshi Mohammad, Wong Sui-Lam

Abstract excerpt

Streptavidin is a versatile molecule for many in vitro and in vivo applications. To optimize the production of the full-length streptavidin in a soluble and functional form via secretion using Bacillus subtilis as the expression host, three different strategies were used. These strategies include the construction of a synthetic streptavidin gene, the installation of a transcription terminator, and the use of a...

Topics

Bacillus subtilis
Biotin
Genetic Vectors
Mass Spectrometry
Mutation
Streptavidin

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Open in PubMed

Identifiers

PMID: 11922750
DOI: 10.1006/prep.2001.1582

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