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Article

Engineered single-chain dimeric streptavidins with an unexpected strong preference for biotin-4-fluorescein.

Proceedings of the National Academy of Sciences of the United States of America - 14 Jun 2005

Aslan Filiz M, Yu Yong, Mohr Scott C, Cantor Charles R

Abstract excerpt

Streptavidin, a homotetrameric protein with extremely tight biotin binding (K(d) < or = 10(-14) M), has been widely used as an affinity reagent. Its utility would be increased by engineering single-chain mutants with a wide spectrum of affinities, more suitable for phage-display and chip technolo...

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PMID: 15939877
DOI: 10.1073/pnas.0503112102
PMCID: PMC1150841

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SCD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-15939877-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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