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Article

Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'.

Journal of biotechnology - 10 Mar 2016

Müller Jakob Michael, Wetzel David, Flaschel Erwin, Friehs Karl, Risse Joe Max

Abstract excerpt

Due to its various applications the protein streptavidin is a highly interesting target for heterologous production. This study focuses on different Escherichia coli-based constructs targeting a high-level expression and secretion of streptavidin to the medium. The effect of various promoters, variants of the target gene, leader sequences and host strains on expression and secretion into the culture broth was...

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PMID: 26820322
DOI: 10.1016/j.jbiotec.2016.01.032

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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