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Article

pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans.

Plasmid - 1 Mar 2002

D'Haeze Wim, Verplancke Christa, Mironov Vladimir, Holsters Marcelle

Abstract excerpt

Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique BamHI restriction site upstream of a promoterless lacZ gene. pMH11 and two derivatives with the...

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PMID: 11982330
DOI: 10.1006/plas.2002.1565

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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