Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes.

Article

Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes.

Acta microbiologica Polonica - 1 Jan 1994

Piekarowicz A, Wegleńska A

Abstract excerpt

The E. coli AP1-200-9 strain for rapid identification of genes encoding restriction and modification enzymes carries a temperature sensitive lacZ gene fused to the damage-inducible dinD locus. A derivative of this strain was constructed that has a wild-type form of this locus which allows for a m...

Topics

Bacterial Proteins
Cloning, Molecular
DNA Modification Methylases
DNA Restriction Enzymes
DNA, Recombinant
Escherichia coli
Escherichia coli Proteins
Lac Operon
Mutation
Phenotype
Plasmids
SOS Response, Genetics
Temperature

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