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Article

Fluorescent indicators for imaging protein phosphorylation in single living cells.

Nature biotechnology - 1 Mar 2002

Sato Moritoshi, Ozawa Takeaki, Inukai Kouichi, Asano Tomoichiro, Umezawa Yoshio

Abstract excerpt

To visualize signal transduction based on protein phosphorylation in living cells, we have developed genetically encoded fluorescent indicators, named phocuses. Two different color mutants of green fluorescent protein (GFP) were joined by a tandem fusion domain composed of a substrate domain for the protein kinase of interest, a flexible linker sequence, and a phosphorylation recognition domain that binds with...

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PMID: 11875431
DOI: 10.1038/nbt0302-287

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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