Article
Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Kim Sung-Bae, Tao Hiroaki
Abstract excerpt
The present protocol demonstrates a new strategy for imaging ligand-triggered protein phosphorylation using circularly permutated luciferases (cpLuc): (1) a luciferase is first fragmented into two segments for creating new N- and C-terminal ends in the hydrophilic region, (2) the original N- and C-terminal ends are circularly permutated and linked via a GS linker, whereas the new ends made by fragmentation are...
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