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Article

Electrostatics significantly affect the stability of designed homeodomain variants.

Journal of molecular biology - 8 Feb 2002

Marshall Shannon A, Morgan Chantal S, Mayo Stephen L

Abstract excerpt

The role of electrostatic interactions in determining the stability of designed proteins was studied by constructing and analyzing a set of designed variants of the Drosophila engrailed homeodomain. Computational redesign of 29 surface positions results in a 25-fold mutant with moderate stability, similar to the wild-type protein. Incorporating helix dipole and N-capping considerations into the design algorithm...

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Identifiers

PMID: 11829512
DOI: 10.1006/jmbi.2001.5326

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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