Article
Rational modification of protein stability by targeting surface sites leads to complicated results.
Proceedings of the National Academy of Sciences of the United States of America - 9 Jul 2013
Xiao Shifeng, Patsalo Vadim, Shan Bing, Bi Yuan, Green David F, Raleigh Daniel P
Abstract excerpt
The rational modification of protein stability is an important goal of protein design. Protein surface electrostatic interactions are not evolutionarily optimized for stability and are an attractive target for the rational redesign of proteins. We show that surface charge mutants can exert stabilizing effects in distinct and unanticipated ways, including ones that are not predicted by existing methods, even when...
Topics
- Amino Acid Sequence
- Binding Sites
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Proteins
- Solubility
- Static Electricity