Article
A phage display selection of engrailed homeodomain mutants and the importance of residue Q50.
Nucleic acids research - 1 Jan 2004
Simon Matthew D, Sato Ken, Weiss Gregory A, Shokat Kevan M
Abstract excerpt
Mutants of engrailed homeodomain (HD) that retain DNA-binding activity were isolated using a phage display selection. This selection was used to enrich for active DNA-binding clones from a complex library consisting of over a billion members. A more focused library of mutant homeodomains consisting of all possible amino acid combinations at two DNA-contacting residues (I47 and Q50) was constructed and screened...
Topics
- Binding Sites
- Consensus Sequence
- Glutamine
- Homeodomain Proteins
- Models, Molecular
- Mutagenesis
- Mutation
- Peptide Library
- Protein Engineering
- Recombinant Fusion Proteins
- Viral Proteins