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Article

A phage display selection of engrailed homeodomain mutants and the importance of residue Q50.

Nucleic acids research - 1 Jan 2004

Simon Matthew D, Sato Ken, Weiss Gregory A, Shokat Kevan M

Abstract excerpt

Mutants of engrailed homeodomain (HD) that retain DNA-binding activity were isolated using a phage display selection. This selection was used to enrich for active DNA-binding clones from a complex library consisting of over a billion members. A more focused library of mutant homeodomains consisting of all possible amino acid combinations at two DNA-contacting residues (I47 and Q50) was constructed and screened...

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Identifiers

PMID: 15247345
DOI: 10.1093/nar/gkh690
PMCID: PMC484177

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