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Article

A refined method for the determination of Saccharomyces cerevisiae cell wall composition and beta-1,6-glucan fine structure.

Analytical biochemistry - 1 Feb 2002

Magnelli Paula, Cipollo John F, Abeijon Claudia

Abstract excerpt

In yeast and other fungi, cell division, cell shape, and growth depend on the coordinated synthesis and degradation of cell wall polymers. We have developed a reliable and efficient micro method to determine Saccharomyces cerevisiae cell wall composition that distinguishes between beta1,3- and beta1,6-glucan. The method is based on the sequential treatment of cell walls with specific hydrolytic enzymes followed...

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PMID: 11811978
DOI: 10.1006/abio.2001.5473

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