Article
Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages.
The Journal of biological chemistry - 27 May 2005
Yin Qing Yuan, de Groot Piet W J, Dekker Henk L, de Jong Luitzen, Klis Frans M, de Koster Chris G
Abstract excerpt
The cell wall of yeast contains proteins that are covalently bound to the glycan network. These cell wall proteins (CWPs) mediate cell-cell interactions and may be involved in cell wall biosynthesis. Using tandem mass spectrometry, we have identified 19 covalently bound CWPs of Saccharomyces cerevisiae. Twelve of them are shown for the first time to be covalently incorporated into the cell wall. The identified...
Topics
- Amino Acid Sequence
- Candida albicans
- Cell Wall
- Glycoproteins
- Glycosylphosphatidylinositols
- Heat-Shock Proteins
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Peptide Fragments