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Article

A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts.

FEMS yeast research - 1 Sept 2014

Schiavone Marion, Vax Amélie, Formosa Cécile, Martin-Yken Hélène, Dague Etienne, François Jean M

Abstract excerpt

A reliable method to determine cell wall polysaccharides composition in yeast is presented, which combines acid and enzymatic hydrolysis. Sulphuric acid treatment is used to determine mannans, whereas specific hydrolytic enzymes are employed in a two sequential steps to quantify chitin and the proportion of β-(1,3) and β-(1,6)-glucan in the total β-glucan of the cell wall. In the first step, chitin and...

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PMID: 25041403
DOI: 10.1111/1567-1364.12182

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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