A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants of Saccharomyces cerevisiae.

doi:10.1002/(SICI)1097-0061(1998100)14:14<1297::AID-YEA310>3.0.CO;2-L

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Article

A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants of Saccharomyces cerevisiae.

Yeast (Chichester, England) - 1 Oct 1998

Dallies N, François J, Paquet V

Abstract excerpt

A reliable acid hydrolysis method for quantitative determination of the proportion of beta-glucan, mannan and chitin in Saccharomyces cerevisiae cell wall is reported together with a simple extraction procedure to quantify within a standard error of less than 2% the proportion of the wall per gra...

Topics

Acids
Cell Wall
Chitin
Glucans
Hydrolysis
Mannans
Monosaccharides
Mutation
Polysaccharides
Saccharomyces cerevisiae
Schizosaccharomyces
Subcellular Fractions

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