Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants.

doi:10.1007/s002530100824

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Article

Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants.

Applied microbiology and biotechnology - 1 Mar 2002

Ha C H, Lim K H, Kim Y T, Lim S T, Kim C W, Chang H I

Abstract excerpt

The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The...

Topics

Alkalies
Cell Wall
Chromatography, Gas
Chromatography, High Pressure Liquid
Glucan Endo-1,3-beta-D-Glucosidase
Glucans
Magnetic Resonance Spectroscopy
Molecular Structure
Mutation
Polysaccharides, Bacterial
Saccharomyces cerevisiae
Solubility

Source

Open in PubMed

Identifiers

PMID: 11935190
DOI: 10.1007/s002530100824

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