Article
Cell wall beta-(1,6)-glucan of Saccharomyces cerevisiae: structural characterization and in situ synthesis.
The Journal of biological chemistry - 15 May 2009
Aimanianda Vishukumar, Clavaud Cécile, Simenel Catherine, Fontaine Thierry, Delepierre Muriel, Latgé Jean-Paul
Abstract excerpt
Despite its essential role in the yeast cell wall, the exact composition of the beta-(1,6)-glucan component is not well characterized. While solubilizing the cell wall alkali-insoluble fraction from a wild type strain of Saccharomyces cerevisiae using a recombinant beta-(1,3)-glucanase followed by chromatographic characterization of the digest on an anion exchange column, we observed a soluble polymer that eluted...
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