Article
Sp3 represses gene expression via the titration of promoter-specific transcription factors.
The Journal of biological chemistry - 22 Mar 2002
Kennett Sarah B, Moorefield K Scott, Horowitz Jonathan M
Abstract excerpt
We have determined previously that Sp3 encodes three distinct gene products as follows: a full-length protein (Sp3) that is an activator of transcription and two isoforms (M1 and M2) derived via internal translational initiation that function as transcriptional repressors. To identify amino acids and functions required for transcriptional repression, we employed PCR-directed mutagenesis to create a panel of...
Topics
- Amino Acids
- Animals
- Binding, Competitive
- Cell Line
- Cell Nucleus
- DNA-Binding Proteins
- Drosophila
- Gene Expression
- Genes, MDR
- Humans
- Insecta
- Mutagenesis, Site-Directed
- Mutation
- Plasmids