Article
Stimulation of the Mu A protein-mediated strand cleavage reaction by the Mu B protein, and the requirement of DNA nicking for stable type 1 transpososome formation. In vitro transposition characteristics of mini-Mu plasmids carrying terminal base pair mutations.
The Journal of biological chemistry - 15 Feb 1991
Surette M G, Harkness T, Chaconas G
Abstract excerpt
We have examined the effects of a T----C point mutation at the terminal nucleotide of the Mu ends in a mini-Mu plasmid on the early steps in the in vitro transposition reaction. These mutations inhibit the introduction of nicks at the Mu ends in a reaction with Mu A, HU, and integration host fact...
Topics
- Adenosine Triphosphate
- Autoradiography
- Bacteriophage mu
- Base Sequence
- DNA Transposable Elements
- DNA, Viral
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Plasmids
- Transposases
- Viral Proteins