Article
N-terminal contributions of the gamma-subunit of fetal hemoglobin to its tetramer strength: remote effects at subunit contacts.
Protein science : a publication of the Protein Society - 1 Jan 2002
Yagami Takeshi, Ballard Barry T, Padovan Julio Cesar, Chait Brian T, Popowicz Anthony M, Manning James M
Abstract excerpt
The greatly increased tetramer strength of liganded fetal hemoglobin compared with adult hemoglobin is shown by its 70-fold smaller tetramer-dimer dissociation constant. This property has been shown previously to be only partially caused by the 5-amino-acid differences at both types of interfaces in each hemoglobin. A major contributor to tetramer strengthening is the 18-amino-acid N-terminal A helix of the...
Topics
- Amino Acid Sequence
- Aspartic Acid
- Circular Dichroism
- Dimerization
- Dose-Response Relationship, Drug
- Fetal Hemoglobin
- Glutamic Acid
- Humans
- Hydrogen-Ion Concentration
- Isoelectric Focusing