Article
Site-directed mutations of human hemoglobin at residue 35beta: a residue at the intersection of the alpha1beta1, alpha1beta2, and alpha1alpha2 interfaces.
Protein science : a publication of the Protein Society - 1 Sept 2001
Kavanaugh J S, Weydert J A, Rogers P H, Arnone A, Hui H L, Wierzba A M, Kwiatkowski L D, Paily P, Noble R W, Bruno S, Mozzarelli A
Abstract excerpt
Because Tyr35beta is located at the convergence of the alpha1beta1, alpha1beta2, and alpha1alpha2 interfaces in deoxyhemoglobin, it can be argued that mutations at this position may result in large changes in the functional properties of hemoglobin. However, only small mutation-induced changes in functional and structural properties are found for the recombinant hemoglobins betaY35F and betaY35A. Oxygen...
Topics
- Amino Acid Substitution
- Binding Sites
- Carbon Monoxide
- Crystallography, X-Ray
- Hemoglobins
- Humans
- Kinetics
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation