Article
Molecular basis for interaction between Icap1 alpha PTB domain and beta 1 integrin.
The Journal of biological chemistry - 8 Mar 2002
Chang David D, Hoang Bao Q, Liu Jenny, Springer Timothy A
Abstract excerpt
Icap1 alpha is a 200-amino acid protein that binds to the COOH-terminal 13 amino acids ((786)AVTTVVNPKYEGK(798)) of the integrin beta(1) subunit. Alanine scanning mutagenesis of this region revealed that Val(787), Val(790), and (792)NPKY(795) are critical for Icap1 alpha binding. The NPXY motif is a known binding substrate for phosphotyrosine binding (PTB) domain proteins. The sequences of Icap1 alpha, residues...
Topics
- 3T3 Cells
- Adaptor Proteins, Signal Transducing
- Alanine
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Binding Sites
- Carrier Proteins
- Cytoplasm
- DNA Mutational Analysis
- Glutathione Transferase
- Humans
- Integrin beta1
- Intracellular Signaling Peptides and Proteins