Article
Metabolic engineering in yeast demonstrates that S-adenosylmethionine controls flux through the methylenetetrahydrofolate reductase reaction in vivo.
The Journal of biological chemistry - 8 Feb 2002
Roje Sanja, Chan Sherwin Y, Kaplan Fatma, Raymond Rhonda K, Horne Donald W, Appling Dean R, Hanson Andrew D
Abstract excerpt
One-carbon flux into methionine and S-adenosylmethionine (AdoMet) is thought to be controlled at the methylenetetrahydrofolate reductase (MTHFR) step. Mammalian MTHFRs are inhibited by AdoMet in vitro, and it has been proposed that methyl group biogenesis is regulated in vivo by this feedback loop. In this work, we used metabolic engineering in the yeast Saccharomyces cerevisiae to test this hypothesis. Like...
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