Article
A mutated hygromycin resistance gene is functional in the n-alkane-assimilating yeast Candida tropicalis.
Archives of microbiology - 1 Mar 2000
Hara A, Ueda M, Misawa S, Matsui T, Furuhashi K, Tanaka A
Abstract excerpt
Development of a transformation system in the n-alkane-assimilating diploid yeast Candida tropicalis requires an antibiotic resistance gene in order to establish a selectable marker. The resistance gene for hygromycin B has often been used as a selectable marker in yeast transformation. However, C. tropicalis harboring the hygromycin resistance gene (HYG) was as sensitive to hygromycin B as the wild-type strain....
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