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New pFA‐cassettes for PCR‐based gene manipulation in <i>Candida albicans</i>

28 Sept 2006

Abstract excerpt

Several modules for efficient PCR-based gene disruption have recently been introduced in Candida albicans. These are based on auxotrophic marker genes for deficient strains derived from SC5314/CAI4. Commonly used protocols for the transformation C. albicans are based either on the lithium acetate procedure or on electroporation also used for Saccharomyces cerevisiae. Here we present our updated arsenal of...

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PMID: 17009297
DOI: 10.1002/jobm.200510133

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