Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.

Nucleic acids research - 1 Nov 2001

Booth C, Griffith E, Brady G, Lydall D

Abstract excerpt

We have developed a method that allows quantitative amplification of single-stranded DNA (QAOS) in a sample that is primarily double-stranded DNA (dsDNA). Single-stranded DNA (ssDNA) is first captured by annealing a tagging primer at low temperature. Primer extension follows to create a novel, ssDNA-dependent, tagged molecule that can be detected by PCR. Using QAOS levels of between 0.2 and 100% ssDNA can be...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Centromere
Share to Chromosomes, Fungal
Share to Cyclin B

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Probes

Share to DNA, Single-Stranded

Share to Genome, Fungal

Share to Telomere

Share to Templates, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 11691929
DOI: 10.1093/nar/29.21.4414
PMCID: PMC60175

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.PMID 11691929Read ↗

Systematic Analysis of the DNA Damage Response Network in Telomere Defective Budding Yeast.Semantic proximity Read ↗

Systematic analysis of the effects of the DNA damage response network in telomere defective budding yeastSemantic proximity Read ↗

Genomic mapping of single-stranded DNA in hydroxyurea-challenged yeasts identifies origins of replication.Semantic proximity Read ↗

Linear chromosome maintenance in the absence of essential telomere-capping proteins.Semantic proximity Read ↗

Survival and Growth of Yeast without Telomere Capping by Cdc13 in the Absence of Sgs1, Exo1, and Rad9Semantic proximity Read ↗

Identification of the single-strand telomeric DNA binding domain of the Saccharomyces cerevisiae Cdc13 protein.Semantic proximity Read ↗

Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance.Semantic proximity Read ↗

Strand-specific ChIP-seq at DNA breaks distinguishes ssDNA versus dsDNA binding and refutes single-stranded nucleosomesSemantic proximity Read ↗

A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae.Semantic proximity Read ↗

Improved short nascent strand sequencing (iSNS-seq) enhances DNA replication origin detection and reduces non-origin biasesSemantic proximity Read ↗

Engineering yeast chromosomal telomeres with a bacteriophage system.Semantic proximity Read ↗

Hypermutability of damaged single-strand DNA formed at double-strand breaks and uncapped telomeres in yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.