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Article

Screens using RNAi and cDNA expression as surrogates for genetics in mammalian tissue culture cells.

Cold Spring Harbor symposia on quantitative biology - 1 Jan 2005

Pearlberg J, Degot S, Endege W, Park J, Davies J, Gelfand E, Sawyer J, Conery A, Doench J, Li W, Gonzalez L, Boyce F M, Brizuela L, Labaer J, Grueneberg D, Harlow E

Abstract excerpt

We have developed methods for the automation of transfection-grade DNA preparation, high-throughput retroviral preparation, and highly parallel phenotypic screens to establish approaches that will allow investigators to examine in an unbiased manner the roles of proteins in mammalian cells. These methods have been used to raise or lower the levels of individual kinases in individual micro-well cultures either by...

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Identifiers

PMID: 16869783
DOI: 10.1101/sqb.2005.70.047

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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