Article
Three-dimensional transgenic cell model to quantify genotoxic effects of space environment.
Advances in space research : the official journal of the Committee on Space Research (COSPAR) - 1 Jan 2001
Gonda S R, Wu H, Pingerelli P L, Glickman B W
Abstract excerpt
In this paper we describe a three-dimensional, multicellular tissue-equivalent model, produced in NASA-designed, rotating wall bioreactors using mammalian cells engineered for genomic containment of multiple copies of defined target genes for genotoxic assessment. Rat 2 lambda fibroblasts, genetically engineered to contain high-density target genes for mutagenesis (Stratagene, Inc., Austin, TX), were cocultured...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.