Article
A role for initiation codon context in chloroplast translation.
The Plant cell - 1 Oct 2001
Esposito D, Hicks A J, Stern D B
Abstract excerpt
To study the role of initiation codon context in chloroplast protein synthesis, we mutated the three nucleotides immediately upstream of the initiation codon (the -1 triplet) of two chloroplast genes in the alga Chlamydomonas reinhardtii. In prokaryotes, the -1 triplet has been proposed to base pair with either the 530 loop of 16S rRNA or the extended anticodon of fMet-tRNA. We found that in vivo, none of the...
Topics
- Animals
- Base Sequence
- Chlamydomonas reinhardtii
- Chloroplasts
- Codon
- Molecular Sequence Data
- Mutation
- Peptide Chain Initiation, Translational
- Plant Proteins
- Protein Biosynthesis
- RNA, Messenger
- RNA, Ribosomal, 16S
- Transformation, Genetic