Article
The initiation codon determines the efficiency but not the site of translation initiation in Chlamydomonas chloroplasts.
The Plant cell - 1 Aug 1995
Chen X, Kindle K L, Stern D B
Abstract excerpt
To study translation initiation in Chlamydomonas chloroplasts, we mutated the initiation codon AUG to AUU, ACG, ACC, ACU, and UUC in the chloroplast petA gene, which encodes cytochrome f of the cytochrome b6/f complex. Cytochrome f accumulated to detectable levels in all mutant strains except the one with a UUC codon, but only the mutant with an AUU codon grew well at 24 degrees C under conditions that require...
Topics
- Animals
- Base Sequence
- Chlamydomonas
- Chloroplasts
- Codon, Initiator
- Cytochromes
- Cytochromes f
- Immunoblotting
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Peptide Chain Initiation, Translational