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Article

Camptothecin resistance from a single mutation changing glycine 363 of human DNA topoisomerase I to cysteine.

Cancer research - 15 Sept 1993

Benedetti P, Fiorani P, Capuani L, Wang J C

Abstract excerpt

A full-length human DNA topoisomerase I complementary DNA clone was mutagenized in vitro and the mutagenized DNA was used to replace wild-type human TOP1 complementary DNA in YCpGAL1-hTOP1, a plasmid constructed for the expression of the human enzyme in yeast. A yeast strain devoid of yeast DNA topoisomerase I and permeable to the anticancer drug camptothecin was transformed with the plasmid pool. Assays of DNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 8395982

Genes

TOP1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Camptothecin resistance from a single mutation changing glycine 363 of human DNA topoisomerase I to cysteine.PMID 8395982Read ↗

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