Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

TDP1 splice-site mutation causes HAP1 cell hypersensitivity to topoisomerase I inhibition.

Nucleic acids research - 11 Jan 2025

Goh Chen Gang, Bader Aldo S, Tran Tuan-Anh, Belotserkovskaya Rimma, D'Alessandro Giuseppina, Jackson Stephen P

Abstract excerpt

HAP1 is a near-haploid human cell line commonly used for mutagenesis and genome editing studies due to its hemizygous nature. We noticed an unusual hypersensitivity of HAP1 to camptothecin, an antineoplastic drug that stabilizes topoisomerase I cleavage complexes (TOP1ccs). We have attributed this hypersensitivity to a deficiency of TDP1, a key phosphodiesterase involved in resolving abortive TOP1ccs. Through...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Camptothecin
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Topoisomerases, Type I

Share to Gene Editing

Share to Phosphoric Diester Hydrolases

Share to RNA Splice Sites

Share to Topoisomerase I Inhibitors

Source

Open in PubMed Open full text

Identifiers

PMID: 39660638
DOI: 10.1093/nar/gkae1163
PMCID: PMC11754736

Genes

TDP1
HAP1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

TDP1 splice-site mutation causes HAP1 cell hypersensitivity to topoisomerase I inhibition.PMID 39660638Read ↗

Transcriptomic Analysis of CRISPR/Cas9-Mediated TDP1-Knockout HEK293A CellsSemantic proximity Read ↗

TDP1 mutation causing SCAN1 neurodegenerative syndrome hampers the repair of transcriptional DNA double-strand breaks.Semantic proximity Read ↗

Nonclassic Functions of Human Topoisomerase I: Genome-Wide and Pharmacologic AnalysesSemantic proximity Read ↗

Epigenetic and genetic inactivation of tyrosyl-DNA-phosphodiesterase 1 (TDP1) in human lung cancer cells from the NCI-60 panel.Semantic proximity Read ↗

Domain interactions affecting human DNA topoisomerase I catalysis and camptothecin sensitivity.Semantic proximity Read ↗

TDP1-independent pathways in the process and repair of TOP1-induced DNA damageSemantic proximity Read ↗

PARP1 regulates the genomic ribonucleotide processing activity of TOP1 to prevent the formation of toxic TOP1-DNA adducts and the associated mutationsSemantic proximity Read ↗

TDP1 overexpression in human cells counteracts DNA damage mediated by topoisomerases I and II.Semantic proximity Read ↗

Heterozygous disruption of the DNA topoisomerase I gene confers cellular resistance to camptothecin in human cells.Semantic proximity Read ↗

SCAN1 mutant Tdp1 accumulates the enzyme--DNA intermediate and causes camptothecin hypersensitivity.Semantic proximity Read ↗

The role of TDP1 from budding yeast in the repair of DNA damage.Semantic proximity Read ↗

Tyrosyl-DNA phosphodiesterase 1 (TDP1) and SPRTN protease repair histone 3 and topoisomerase 1 DNA-protein crosslinks <i>in vivo</i>Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.