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Article

Alterations in linker flexibility suppress DNA topoisomerase I mutant-induced cell lethality.

The Journal of biological chemistry - 30 Mar 2007

Losasso Carmen, Cretaio Erica, Palle Komaraiah, Pattarello Luca, Bjornsti Mary-Ann, Benedetti Piero

Abstract excerpt

Eukaryotic DNA topoisomerase I (Top1p) catalyzes changes in DNA topology via the formation of a covalent enzyme-DNA intermediate, which is reversibly stabilized by the anticancer agent camptothecin (CPT). Crystallographic studies of the 70-kDa C terminus of human Top1p bound to duplex DNA describe a monomeric protein clamp circumscribing the DNA helix. The structures, which lack the N-terminal domain, comprise...

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Identifiers

PMID: 17276985
DOI: 10.1074/jbc.M608200200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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