Article
Detection of errors in dinucleotide repeat typing by nondenaturing electrophoresis.
Electrophoresis - 1 Aug 2001
Rodríguez S, Visedo G, Zapata C
Abstract excerpt
This study shows that consideration of minor bands (heteroduplex, shadow, and faint bands) associated with allele bands in nondenaturing polyacrylamide gel electrophoresis (PAGE) after polymerase chain reaction (PCR) is effective for detecting PCR processing errors that lead to mistyping of heterozygotes as homozygotes. Notably, we show that minor bands in native gels are highly effective for detecting allele...
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