Article
A study of the origin of 'shadow bands' seen when typing dinucleotide repeat polymorphisms by the PCR.
Human molecular genetics - 1 Apr 1993
Hauge X Y, Litt M
Abstract excerpt
Dinucleotide repeat polymorphisms ('microsatellites') are usually typed by resolving the products of PCR amplification on denaturing acrylamide gels. With this methodology, an allele consists not of a single fragment, but rather of a ladder of fragments, typically separated by intervals of 2nt. Mechanisms that have been invoked to explain the generation of these 'shadow bands' include slipped strand mispairing...
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