Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Intrinsic biocontainment: multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes.

Proceedings of the National Academy of Sciences of the United States of America - 10 Feb 2015

Cai Yizhi, Agmon Neta, Choi Woo Jin, Ubide Alba, Stracquadanio Giovanni, Caravelli Katrina, Hao Haiping, Bader Joel S, Boeke Jef D

Abstract excerpt

Biocontainment may be required in a wide variety of situations such as work with pathogens, field release applications of engineered organisms, and protection of intellectual properties. Here, we describe the control of growth of the brewer's yeast, Saccharomyces cerevisiae, using both transcriptional and recombinational "safeguard" control of essential gene function. Practical biocontainment strategies dependent...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biotechnology
Share to Genes, Essential

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Engineering

Share to Organisms, Genetically Modified

Share to Recombinases

Share to Recombination, Genetic

Share to Saccharomyces cerevisiae

Share to Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 25624482
DOI: 10.1073/pnas.1424704112
PMCID: PMC4330768

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Intrinsic biocontainment: multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes.PMID 25624482Read ↗

Engineering stringent genetic biocontainment of yeast with a protein stability switchSemantic proximity Read ↗

Engineering stringent genetic biocontainment of yeast with a protein stability switchSemantic proximity Read ↗

Functional characterisation of an essential neo-chromosome III in Sc2.0 strain reveals opportunities and challenges for genome minimisation in Sc3.0Semantic proximity Read ↗

Regulated ectopic expression and allelic-replacement mutagenesis as a method for gene essentiality testing in Staphylococcus aureus.Semantic proximity Read ↗

Direct Isolation of Seamless Mutant Bacterial Artificial Chromosomes.Semantic proximity Read ↗

Gene drive that results in addiction to a temperature sensitive version of an essential gene triggers population collapse in DrosophilaSemantic proximity Read ↗

Superloser: a plasmid shuffling vector for <i>Saccharomyces cerevisiae</i> with exceedingly low backgroundSemantic proximity Read ↗

A high-efficiency method to replace essential genes with mutant alleles in yeast.Semantic proximity Read ↗

The dawn of active genetics.Semantic proximity Read ↗

Rapid, scalable, combinatorial genome engineering by Marker-less Enrichment and Recombination of Genetically Engineered loci (MERGE)Semantic proximity Read ↗

Yeast chromosomal engineering to improve industrially-relevant phenotypes.Semantic proximity Read ↗

Evolverator: An engineered yeast system to drive rapid continuous <i>in vivo</i> evolution of proteinsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.