Article
Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system.
Yeast (Chichester, England) - 15 Sept 1998
Bellí G, Garí E, Aldea M, Herrero E
Abstract excerpt
A promoter-substitution cassette has been constructed that allows one-step substitution of chromosomal gene promoters for the tetracycline-regulatable tetO promoter in yeast cells, which uses kanMX4 as selective marker for geneticin resistance. Oligonucleotides for PCR amplification of the casset...
Topics
- Cell Division
- Gene Expression Regulation, Fungal
- Genes, Essential
- Genes, Fungal
- Mutagenesis, Insertional
- Mutation
- Open Reading Frames
- Phenotype
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Tetracycline
- Tetracycline Resistance