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Article

Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system.

Yeast (Chichester, England) - 15 Sept 1998

Bellí G, Garí E, Aldea M, Herrero E

Abstract excerpt

A promoter-substitution cassette has been constructed that allows one-step substitution of chromosomal gene promoters for the tetracycline-regulatable tetO promoter in yeast cells, which uses kanMX4 as selective marker for geneticin resistance. Oligonucleotides for PCR amplification of the cassette are designed to allow homologous recombination through short flanking regions of homology with the upstream...

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PMID: 9778798
DOI: 10.1002/(SICI)1097-0061(19980915)14:12<1127::AID-YEA300>3.0.CO;2-#

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