Attenuation of Murray Valley encephalitis virus by site-directed mutagenesis of the hinge and putative receptor-binding regions of the envelope protein.

Abstract excerpt

Molecular determinants of virulence in flaviviruses cluster in two regions on the three-dimensional structure of the envelope (E) protein; the base of domain II, believed to serve as a hinge during pH-dependent conformational change in the endosome, and the lateral face of domain III, which contains an integrin-binding motif Arg-Gly-Asp (RGD) in mosquito-borne flaviviruses and is believed to form the...

Topics

• Animals
• Binding Sites
• Chlorocebus aethiops
• Encephalitis Virus, Murray Valley
• Encephalitis, Arbovirus
• Mutagenesis, Site-Directed
• Mutation
• Receptors, Virus
• Vero Cells
• Viral Envelope Proteins
• Virus Replication