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Article

A rapid screen for putative mislocalization mutations by using a GAL4-hybrid protein.

Molecules and cells - 30 Jun 2001

Lee M Y, Kim J

Abstract excerpt

A GAL4 one-hybrid system was used to develop an indirect assay for intracellular localization. The Kar1 protein, a component of the yeast spindle pole body (SPB), was shown to be targeted to the SPB by electron microscopic analysis, or indirect immunofluorescence methods. To assay localization of the Kar1p by measuring the reporter gene expression on solid media, we constructed Kar1-Gal4 hybrid proteins with or...

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PMID: 11459215

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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