Article
Retroviral transduction of alveolar bone cells with a temperature-sensitive SV40 large T antigen.
Cell and tissue research - 1 Jun 2001
Salih V, Knowles J C, O'Hare M J, Olsen I
Abstract excerpt
We have transduced adult human alveolar bone (AB) cells with a gene construct encoding a temperature-sensitive mutation of the SV40 large T antigen (tsT). Such cells divided rapidly, for more than 50 passages thus far, at a permissive low temperature (34.5 degrees C), comparable to the non-transduced parental cells at 37 degrees C. However, the tsT-transduced AB cells failed to grow at a non-permissive high...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.