Article
Scleroderma-derived human fibroblasts retain abnormal phenotypic and functional characteristics following retroviral transduction with the SV40 tsT antigen.
Experimental cell research - 1 Oct 1995
Xu S, Vancheeswaran R, Bou-Gharios G, O'Hare M J, Olsen I, Abraham D, Black C
Abstract excerpt
In this study an amphotropic retrovirus has been used to efficiently transduce normal human (NF) and scleroderma (systemic sclerosis; SSc) dermal fibroblasts (SScF) with a sequence encoding a temperature-sensitive mutant of the SV40 large T antigen (tsA58-U19). From the primary outgrowths of skin explants, cultures were generated whose growth was stringently temperature-dependent. When grown at a low, permissive...
Topics
- Antibodies
- Antibody Specificity
- Antigens, Polyomavirus Transforming
- Cell Adhesion
- Cell Adhesion Molecules
- Cell Cycle
- Cell Division
- Cells, Cultured
- Collagen
- Fibroblasts