Article
SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research - 1 Feb 2002
Darimont Christian, Avanti Ornella, Tromvoukis Yvonne, Vautravers-Leone Patricia, Kurihara Nori, Roodman G David, Colgin Lorel M, Tullberg-Reinert Heide, Pfeifer Andrea M A, Offord Elizabeth A, Mace Katherine
Abstract excerpt
In most human primary bone cells, SV40 T-antigen expression was able to expand life span for a few passages before cells undergo growth arrest, described as crisis. In this study, telomerase activity was reconstituted in human osteoblast precursors (hPOB cells) and marrow stromal cells (Saka cells) transformed with the SV40 T antigen. Bone cells with telomerase activity were able to bypass crisis and show...
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