Article
Expression of type X collagen and matrix calcification in three-dimensional cultures of immortalized temperature-sensitive chondrocytes derived from adult human articular cartilage.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Mar 1998
Oyajobi B O, Frazer A, Hollander A P, Graveley R M, Xu C, Houghton A, Hatton P V, Russell R G, Stringer B M
Abstract excerpt
Chondrocytes isolated from normal adult human articular cartilage were infected with a retroviral vector encoding a temperature-sensitive mutant of the simian virus 40 large tumor antigen and a linked geneticin (G418)-resistance marker. G418-resistant colonies were then isolated, ring-cloned, and...
Topics
- Adult
- Anti-Bacterial Agents
- Calcification, Physiologic
- Calcium
- Cartilage, Articular
- Cell Line
- Cell Survival
- Chondrocytes
- Collagen
- Drug Resistance, Microbial
- Gentamicins
- Glycosaminoglycans
- Humans