Article
Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli.
Journal of bacteriology - 1 Jan 1993
Washburn B K, Kushner S R
Abstract excerpt
The loss of DNA helicase II (UvrD) in Escherichia coli results in sensitivity to UV light and increased levels of spontaneous mutagenesis. While the effects of various uvrD alleles have been analyzed in vivo, the proteins produced by these alleles have not been examined in any detail. We have clo...
Topics
- Adenosine Triphosphatases
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Helicases
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Escherichia coli Proteins
- Gene Deletion
- Genes, Bacterial
- Genetic Vectors
- Kinetics
- Magnesium Chloride
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Insertional
- Oligodeoxyribonucleotides