Article
Reduced allele dropout in single-cell analysis for preimplantation genetic diagnosis of cystic fibrosis.
Journal of assisted reproduction and genetics - 1 Feb 1996
Ray P F, Winston R M, Handyside A H
Abstract excerpt
BACKGROUND: For couples at risk of transmitting a known single-gene defect, preimplantation genetic diagnosis (PGD) allows the identification and transfer of only unaffected embryos following in vitro fertilisation (IVF), single-cell biopsy at about the eight-cell stage, and genetic analysis by P...
Topics
- Alleles
- Artifacts
- Blastomeres
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA Mutational Analysis
- DNA Primers
- Diagnostic Errors
- Female
- Heterozygote
- Humans
- Microchemistry
- Polymerase Chain Reaction
- Predictive Value of Tests
- Pregnancy
- Prenatal Diagnosis
- Sensitivity and Specificity
- Sequence Deletion