Article
Large-scale, saturating insertional mutagenesis of the mouse genome.
Proceedings of the National Academy of Sciences of the United States of America - 4 Sept 2007
Gragerov Alexander, Horie Kyoji, Pavlova Maria, Madisen Linda, Zeng Hongkui, Gragerova Galina, Rhode Alex, Dolka Io, Roth Patricia, Ebbert Amanda, Moe Stephanie, Navas Christopher, Finn Eric, Bergmann John, Vassilatis Demetrios K, Pavlakis George N, Gaitanaris George A
Abstract excerpt
We describe the construction of a large-scale, orderly assembly of mutant ES cells, generated with retroviral insertions and having mutational coverage in >90% of mouse genes. We also describe a method for isolating ES cell clones with mutations in specific genes of interest from this library. This approach, which combines saturating random mutagenesis with targeted selection of mutations in the genes of...
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