Article
Role of glutathione S-transferase mu (GSTM1) in styrene-7,8-oxide toxicity and mutagenicity.
Environmental and molecular mutagenesis - 1 Jan 2001
Shield A J, Sanderson B J
Abstract excerpt
In the human glutathione S-transferase (GST) mu gene family, homozygous deletion of GSTM1 is the null phenotype (frequency of approximately 50% in Caucasians). In the current study, GSTM1 status was determined in human cell lines using reverse transcriptase, polymerase chain reaction, and immunochemistry. Cell lines were challenged with a range of doses of styrene-7,8-oxide (SO) and then toxicity and genotoxicity...
Topics
- Cell Line
- DNA
- Dose-Response Relationship, Drug
- Epoxy Compounds
- Genotype
- Glutathione Transferase
- Humans
- Immunohistochemistry
- Inhibitory Concentration 50
- Mutagens
- Mutation
- Phenotype
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction