Article
Mutants in the ADP-ribosyltransferase cleft of cholera toxin lack diarrheagenicity but retain adjuvanticity.
The Journal of experimental medicine - 7 Apr 1997
Yamamoto S, Takeda Y, Yamamoto M, Kurazono H, Imaoka K, Yamamoto M, Fujihashi K, Noda M, Kiyono H, McGhee J R
Abstract excerpt
Cholera toxin (CT), the most commonly used mucosal adjuvant in experimental animals, is unsuitable for humans because of potent diarrhea-inducing properties. We have constructed two CT-A subunit mutants, e.g., serine-->phenylalanine at position 61 (S61F), and glutamic acid-->lysine at 112 (E112K)...
Topics
- Adjuvants, Immunologic
- Animals
- CD4-Positive T-Lymphocytes
- CHO Cells
- Cholera Toxin
- Cricetinae
- Cyclic AMP
- Cytokines
- Diarrhea
- Dose-Response Relationship, Drug
- Ileum
- Lymphocyte Activation
- Mice
- Mice, Inbred C57BL