Article
Initiation of strand incision at G:T and O(6)-methylguanine:T base mismatches in DNA by human cell extracts.
Nucleic acids research - 1 Jun 2001
Lari S U, Day R S, Dobler K, Paterson M C
Abstract excerpt
Extracts of the human glioma cell line A1235 (lacking O(6)-methylguanine-DNA methyltransferase) are known to restore a G:T mismatch to a normal G:C pair in a G:T-containing model (45 bp) DNA substrate. Herein we demonstrate that substitution of G:T with O(6)-methylguanine:T (m6G:T) results in extract-induced intra-strand incision in the DNA at an efficiency comparable to that of complete repair of the...
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